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Fonds de recherche sur la santé

Université de Sherbrooke Rnomics Platform (animal/human – CMDO preferential rates)

Name of entity

Université de Sherbrooke Rnomics Platform (animal/human - CMDO preferential rates)

Entity type

Plateform

Keywords

PCR, qRT-PCR, Digital PCR, Next Generation Sequencing, NGS, Illumina Miseq, Illumina Nextseq 500, RNA, gene expression, alternative splicing, bioinformatics, genomics, rnomics, absolute quantification.

Area of expertise

The Université de Sherbrooke RNomics Platform provides a wide range of RT-PCR services (qPCR, ASPCR, ddPCR), Next-Generation Sequencing, and bioinformatics services to the research community. We have developed an automated computational and experimental platform for large scale RT-PCR based investigations. Primary applications are for comparative gene expression studies using quantitative real-time RT-PCR (qRT-PCR), and for the annotation and analysis of alternative splicing using endpoint PCR coupled with microcapillary electrophoresis (ASPCR). We routinely design, execute and analyze thousands of PCR reactions per day. Our sequencing service is complete from library preparation, sequencing run, to data analysis and presentation. We offer a mid-throughput service range using Illumina MiSeq and NextSeq 500 platforms. Needs for deeper sequencing are referred to partner genome centers for the sequencing run itself, while we prevail on the library preparation and data analysis. Associated services include RNA and DNA isolation and quality control from cell culture and tissue sources, and extensive bioinformatics support for experimental design and data analysis. Clients can access their results and all experimental data via a secure web portal, where several computational modules are available to optimize data analysis and presentation. The Platform’s expert staff is also available for direct consultation at all stages of each project. The Platform is continually developing new assays and bioinformatics protocols to meet the needs of its expanding client base.

Services offered

  • RNA and DNA isolation and quality control from cell culture and tissue sources.
  • RNA integrity (Agilent Bioanalyser), quantification and reverse transcription.
  • Next-generation sequencing complete service from library preparation to data analysis on Illumina MiSeq et NextSeq 500 platforms.
  • Digital droplet PCR (ddPCR) on Bio-Rad QX200 platform.
  • Quantitative PCR (qPCR) on Bio-Rad CFX platforms (96 and 384 wells).
  • End-point PCR with microcapillary electrophorese detection.
  • Pathogenic microorganism detection for animal facility.
  • Bioinformatique development.
  • PCR primer design system.
  • Statistical analysis.

Responsable

Platform Administration
rnomics-fmss@usherbrooke.ca
819-821-8000 ext. 70455
http://rnomics.med.usherbrooke.ca

Address

RESPONSIBLE NAMES :
Mathieu Durand mathieu.durand@usherbrooke.ca

Elvy Lapointe elvy.lapointe@usherbrooke.ca

Philippe Thibault Philippe.Thibault2@USherbrooke.ca

Rnomics Platform
PRAC, Z8-3036
Faculté de médecine et des sciences de la santé
Université de Sherbrooke
3201 rue Jean-Migneault
Sherbrooke, Québec
J1E 4K8