Université de Sherbrooke RNomics Platform (animal/human)

Name of entity

Université de Sherbrooke RNomics Platform

Entity type

Platform

Keywords

PCR, qRT-PCR, Digital PCR, Next Generation Sequencing, NGS, Illumina Miseq, Illumina Nextseq 500, RNA, gene expression, alternative splicing, bioinformatics, genomics, rnomics, absolute quantification.

Area of expertise

RNomique Platform — Unlock the Full Power of Sequencing and Transcriptomics

Based at the Université de Sherbrooke, the RNomique platform is a center of excellence in functional genomics and transcriptomics. We offer a comprehensive suite of next-generation sequencing (NGS) services—including short- and long-read technologies, advanced bioinformatics, and high-throughput PCR—serving academic, clinical, and industrial partners.

Our cutting-edge technology infrastructure includes:
-Two PromethION P2 sequencers (Oxford Nanopore) for long-read sequencing, ideal for full-length transcript analysis, alternative splicing, isoform structure, plasmid validation, epigenomics, and direct RNA sequencing,
-Two Aviti sequencers (Element Biosciences) offering cost-effective, high-performance short-read sequencing, perfectly suited for RNA-seq, transcriptomics, microbiome profiling, and targeted genomics.

Services offered

• We also provide specialized sequencing services such as:
  -Full plasmid sequencing, with sample drop-off points available across Quebec,
  -CRISPR-Cas9 edit validation,
  -Multiplexed direct RNA sequencing,
  -Metagenomic profiling of microbial communities and antimicrobial resistance genes.Complementing our sequencing capabilities, we operate a fully automated high-throughput PCR platform, capable of processing thousands of reactions daily, including:
  -qPCR with dual-labeled probes or SYBR Green,
  -ASPCR for detailed annotation of alternative splicing,
  -ddPCR for absolute quantification.Our team also provides RNA and DNA extraction and quality control, as well as custom bioinformatics support from experimental design to data interpretation.All results are delivered through a secure, web-based interface featuring interactive visualization and analysis tools.Constantly evolving, the RNomique platform continuously develops innovative protocols and services to meet the growing needs of biomedical and translational research.

  RNomique is more than just a service platform — it’s your strategic partner in genomics and transcriptomics.
  Offered services :  Summary of services offered – RNomics platform
   Next-generation sequencing (NGS):

  Long reads (Oxford Nanopore – PromethION P2):

  -Native RNA sequencing (direct RNA-seq), multiplexing
  -Analysis of alternative splicing and complete isoforms
  -Plasmid validation and circular map construction
  -Epigenetic profiling without amplification (methylation)
  -Targeted enrichment with probes (e.g., CRISPR, splicing, cancer)

  Short reads (Element Aviti x2):

  -RNA-seq (bulk, polyA, rRNA-depleted, total)
  -Targeted and amplicon sequencing
  -Microbiome (16S, shotgun)
  -CRISPR validation, custom panels
  Low-cost multiplexed sequencing

   Sample preparation:

  -RNA and DNA extraction and purification (cultures, tissues, blood, etc.)
  -Quality control (quantification, integrity, purity)
  – Illumina, ONT, Aviti library construction

   Automated high-throughput PCR:

  -qPCR (SYBR Green, TaqMan probes)
  -ASPCR (fine annotation of alternative splicing)
  -ddPCR (absolute quantification)
  -Primer design and validation

   Customized bioinformatics analysis:

  -Experimental design and upstream consulting
  -Data processing, annotation and visualization
  -Customized pipelines for transcriptomics, isoforms, microbiome, CRISPR editing, etc.
  -Secure web portal for results consultation (graphs, tables, reports)

   Support and guidance:

  -Advice at every stage of the project
  -Academic, clinical and industrial services
  -Rapid access and direct assistance from our scientific team

Responsable

Mathieu Durand, Operations Manager, Laboratory Manager
rnomics-fmss@usherbrooke.ca
819-821-8000 ext. 70455
https://rnomics.med.usherbrooke.ca/welcome

Address

RESPONSIBLE NAMES :
Mathieu Durand mathieu.durand@usherbrooke.ca

Maxime Duval maxime.duval2@usherbrooke.ca

Sonia Couture sonia.couture2@usherbrooke.ca

Institut de recherche sur le cancer de l’Université de Sherbrooke (IRCUS)
3201, rue Jean-Mignault local Z8-3036
Sherbrooke, Quebec, Canada
J1E 4K8
Bureau: 75873