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Fonds de recherche sur la santé

Metabolomics platform – MHI (animal/human)

Name of entity

Metabolomics Platform - Montreal Heart Institute (MHI) (fundamental/clinical)

Entity type

Platform of collaboration and service: Mass Spectrometry / Metabolomics

Keywords

Metabolism, metabolomics, targeted and untargeted lipidomics, metabolic flux, stable
isotopes, mass spectrometry, GC-MS, LC-MS, LC-QQQ, LC-QTOF.

Area of expertise

Our primary research focus is on cardiovascular, cardiometabolic, mitochondrial and inflammatory
diseases. We can do absolute and/or relative quantitative, targeted or untargeted, analyses of
metabolites, including their labeling with carbon 13 or deuterium) both in fundamental and clinical
projects. We also analyze various classes of metabolites in different kind of biological matrices such
as plasma, serum, red blood cells, cells, mitochondria, or various animal tissues (heart, liver, muscle,
brain and more) and other species (ex: C. elegans) depending on your needs.

Services offered

1) Available analysis using validated methods.

Untargeted analyses (for biomarker discovery):

  • Semi-quantitative lipidomic analysis (>1,000 entities) by LC-QTOF.

Targeted analyses:

  • Quantitative analysis of amino and organic acids by GC-MS (32 metabolites).
  • Quantitative analysis of fatty acids including saturated, monounsaturated, polyunsaturated (omega-3 and omega-6) and very long chain fatty acids by GC-MS, in specific fractions (phospholipids and triglycerides) on demand (22 metabolites).
  • Semi-quantitative analysis of acylcarnitines by LC-QQQ (>100 species).
  • Quantitative analysis of glycerol, glycerate et glyceraldehyde by GC-MS.
  • Quantitative analysis of metabolites stemming from glucose metabolism such as phosphate sugars and UDP-N-acetyl-glucosamine by LC-QTOF.
  • Semi-quantitative analysis of bile acids by LC-QQQ (11 metabolites).
  • Metabolic flux analysis related to energy metabolism (glycolysis; substrate oxidation)

2) Method development on demand.

3) Scientific support (study design and data interpretation).

Directeur

Director : Christine Des Rosiers, Ph.D. and co-director : Matthieu Ruiz, Ph.D.

Responsable

Isabelle Robillard Frayne, M.Sc. and Caroline Daneault, M.Sc.
metabolomique.icm@mhi-rc.org
514 376-3330 poste 2133
https://www.icm-mhi.org/en/research/labs/fundamental-laboratories/metabolomics

Address

Metabolomics Platform – Montreal Heart Institute (MHI)
Montreal Heart Institute
Research Center, S-3730
5000, Bélanger street
Montreal, Québec
H1T 1C8