Metabolomics Core facility – CRCHUM (animal/human)

Name of entity

Metabolomics Core facility - CRCHUM

Entity type

Platform

Keywords

Metabolomics, metabolite, metabolism, bioanalysis, HPLC, mass spectrometry

Area of expertise

Our mission is to offer metabolite quantification services in tissues, cells and body fluids based on mass spectrometry (targeted metabolomics). Over the years, we have developed expertise focused on metabolic diseases such as obesity and type 2 diabetes. We can quantify simultaneously various key metabolites extracted from different tissues. Our assays include procedures & methods for the determination of energetic equivalents (ATP, NADH, etc.), organic acids (citrate, malate, etc.), amino acids, short-chain fatty acids, bile acids and neurotransmitters.

Services offered

Relative and absolute quantification of metabolites (targeted metabolomics) in tissues, cell samples and biological fluids by mass spectrometry. Assessed metabolites are listed below.

Development of custom methods.

Consultation and project design in metabolomics, as well as full assistance in the interpretation of data, drafting application for funding and publications.

Our technical services are modular. Customers can participate at various steps to reduce costs: sample preparation, HPLC, results analysis, etc.

Our services are offered to the community of the CHUM Research Centre, the MDRC, the CMDO and other academic and industrial institutions.

List of metabolites available

Nucleotides and their derivatives: adenosine, AMP, cAMP, ADP, ATP, GMP, cGMP, GDP, GTP, NAD, NADH, NADP, NADPH

Short-chain acyl-CoAs: acetyl-CoA, malonyl-CoA, acetoacetyl-CoA, HMG-CoA

Organic acids and Krebs cycle derivatives: citrate/isocitrate, malate, succinate, alpha-ketoglutarate, fumarate, oxaloacetate, lactate, pyruvate, acetoacetate

Short-chain fatty acids: acetic, propionic, butyric, isobutyric, valeric, isovaleric, caproic acids

Bile acids: cholic (CA), deoxycholic (DCA), chenodeoxycholic (CDCA), ursodeoxycholic (UDCA), glycodeoxycholic (GDCA), glycoursodeoxycholic (GUDCA), taurocholic (TCA), taurodeoxycholic (TDCA), taurochenodeoxycholic (TCDCA), lithocholic (LCA), α-, β-, ω-muricholic (α-MCA, β-MCA, ω-MCA) acids

Amino acids and their derivatives: alanine, arginine, asparagine, carnitine, citrulline, cystine, glycine, glutamate,
glutamine, histidine, isoleucine, leucine, lysine, methionine, ornithine, phenylalanine, proline, serine, taurine, threonine, tryptophane, tyrosine, valine

Neurotransmitters : acetylcholine, epinephrine, norepinephrine, dopamine, GABA, glutamate, glycine, serotonin

Others: oxidized and reduced glutathione; DHAP, glycerol-3-phosphate, 2-phosphoglycolate

julien.lamontagne.chum@ssss.gouv.qc.ca
(514) 961-4137
https://www.chumontreal.qc.ca/en/crchum/core-facilities-services/metabolomics

Address

Julien Lamontagne, PhD
Supervisor, Metabolomics Core Facility
CRCHUM / Montreal Diabetes Research Center
Pavillon R, room R12-456
900, St-Denis
Montréal, QC, H2X 0A9